Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
pH and Temperature effects on the chimeric Cel5A-Dockerin. The optimum pH and temperature of Cel5A and Cel5A-Dockerin was tested to measure the functional impact of grafting a fungal dockerin domain onto Cel5A. While previous experiments showed that the Cel5A-Dockerin was not significantly different in activity than Cel5A at 80 °C and pH 5, there was a slight narrowing of the optimum pH and temperature of the enzyme. Values shown are the average of three blank-subtracted replicates.
