Table 3.
Strains and plasmids used in this study.
| Strain or Plasmid | Relevant genotype or features | Source |
|---|---|---|
| Strain | ||
| E. coli BL21 (DE3) |
fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5 |
Laboratory stock |
| E. coli Tuner (DE3) | F– ompT hsdSB (rB– mB–) gal dcm lacY1 (DE3) | Laboratory stock |
| Piromyces finnis | [16] | |
| Plasmid | ||
| pET32a | E. coli expression vector to create N-terminal genetic fusion to TrxA | Novagen |
| pET32a-Cel5A | DNA encoding T. maritima Cel5A in pET32a | This study |
| pET32a-Cel5A-Dockerin | DNA encoding T. maritima Cel5A fused to C-terminal dockerin from P. finnis in pET32a | This study |
| pET32a-XynA | DNA encoding T. maritima XynA in pET32a | This study |
| pET32a-XynA-Dockerin | DNA encoding T. maritima XynA fused to C-terminal dockerin from P. finnis in pET32a | This study |