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. 2020 Jan 15;29(3):495–505. doi: 10.1093/hmg/ddz319

Figure 5.

Figure 5

Loss of function mutant of sut-2 suppresses induced motility defects in both tau-Tg and Aβ1-42;tau-Tg worms. After 24-h temperature upshift at 25°C, day-one adult worms were subjected to a swimming assay to assess motility. One-minute videos were captured and subsequently analyzed by automated WormTracker software counting the number of body bends per minute. (A) Suppression of tau phenotype by a sut-2 loss of function mutation was observed in tau-Tg background, where tau-Tg worms showed a mean of 44.9 ± 3.0 SEM body bends per minute versus tau-Tg;sut-2 lof worms that showed a mean of 174.5 ± 7.4 SEM body bends per minute. (B) Notably, Aβ1-42;tau-Tg worms showed a mean of 24.09 ± 3.5 SEM while Aβ1-42;tau-Tg;sut-2 lof showed a partial rescue of tau-induced motility defects with a mean of 72.6 ± 5.0 SEM body bends per minute. For reference, non-transgenic worms typically have a mean of approximately 150-200 body bends per minute. (N = 2, n ≥ 50, unpaired t-test. Error bars represent SEM. ***P < 0.0001).