Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 1;577(7788):95–102. doi: 10.1038/s41586-019-1817-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 10 — a, Serial FDG PET–CT scans at 2 and 4 weeks after BCG vaccination showed increased metabolism (surrogate for inflammation) localized to the lung LNs (green arrows), lung lobes and spleen (yellow arrow) elicited by the IV but not by other routes (cohort 5a, b, n = 2 macaques). Warm colours indicate increased FDG retention; scale represents standardized uptake values. NHP ID numbers are listed above each scan; ‘H’ denotes the heart. b, Spleen volume was calculated from CT scans at 2 and 4 weeks after BCG vaccination (n = 2 macaques). At these time points, animals given IV BCG had approximately twofold larger spleens than those given ID BCG, with AE/ID BCG NHPs also displaying modestly enlarged spleens. c, Thoracic LNs were measured at necropsy, 4 weeks after BCG vaccination (n = 2 macaques); LNs from IV BCG NHPs were enlarged compared to those from ID_low NHPs. Kruskal–Wallis test was run; Dunn’s adjusted P values are reported comparing each vaccine group to the ID_low group. d, e, H&E-stained sections of thoracic LNs from vaccinated NHPs (n = 2 macaques), 4 weeks after BCG vaccination. d, General structure with respect to cortical and medullary architecture and appearance was normal in LNs from ID_low, ID_high and AE/ID vaccinated NHPs. The thoracic LNs from the IV-vaccinated macaques demonstrated marked follicular lymphoid hyperplasia, with enlarged, prominent, variably sized follicles, often with active, expanded germinal centres. Original magnification, ×4. e, Small, non-necrotizing epithelioid histiocytic aggregates (non-necrotizing granulomas, black arrows) were abundantly disseminated within thoracic LNs from the IV BCG macaques. In the AE/ID NHPs, a wide nodal distribution of such lesions was also seen, although granuloma numbers and density were substantially less. The ID_high NHPs had only one observable granuloma in a single thoracic LN and in the ID_low NHPs, no such structures were evident. Original magnification, ×10.

Source Data