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. 2020 Feb 6;8:44. doi: 10.3389/fbioe.2020.00044

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Copyright © 2020 Wong, Gonzalez-Miro, Sutherland-Smith and Rehm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural characterization of various NPP-Ss. (A) SEM and TEM micrographs of plain NPP-S. Black scale bar, 1 μm; white scale bar, 100 nm. (B) Particle size distribution of plain NPP-S by DLS analysis (mean, n = 3). (C) Particle size distribution of SpGFP-H6 and SnGFP-H6 immobilized NPP-Ss by DLS analysis (mean, n = 3) using particle size distribution of plain NPP-S determined in B as negative control and PhaC-GFP-S as positive control. (D) Particle size distribution of SpBLA-H6 and SnBLA-H6 immobilized NPP-Ss by DLS analysis (mean, n = 3) using particle size distribution of plain NPP-S determined in B as negative control and BLA-PhaC-S as positive control. (E) Particle size distribution of various dual-functionalized NPP-Ss by DLS analysis (mean, n = 3) using particle size distribution of plain NPP-S determined in B as negative control. NPP-S, NPP fusion protein-displaying PHA spheres; WT-S, WT-displaying PHA spheres; NPP-SpGFP-S, NPP-SpGFP ligated protein-displaying PHA spheres; SnGFP-NPP-S, SnGFP-NPP ligated protein-displaying PHA spheres; PhaC-GFP-S, PhaC-GFP fusion protein-displaying PHA spheres; NPP-SpBLA-S, NPP-SpBLA ligated protein-displaying PHA spheres; SnBLA-NPP-S, SnBLA-NPP ligated protein-displaying PHA spheres; BLA-PhaC-S, BLA-PhaC fusion protein-displaying PHA spheres; SnGFP-NPP-SpGFP-S, SnGFP-NPP-SpGFP ligated protein-displaying PHA spheres; SnBLA-NPP-SpBLA-S, SnBLA-NPP-SpBLA ligated protein-displaying PHA spheres; SnBLA-NPP-SpGFP-S, SnBLA-NPP-SpGFP ligated protein-displaying PHA spheres; SnGFP-NPP-SpBLA-S, SnGFP-NPP-SpBLA ligated protein-displaying PHA spheres.