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. 2020 Feb 6;8:44. doi: 10.3389/fbioe.2020.00044

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Copyright © 2020 Wong, Gonzalez-Miro, Sutherland-Smith and Rehm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exposure of plain NPP-S to extreme conditions prior functionalization as visualized by SDS-PAGE analysis and screening of samples under blue light. (A) NPP-S could immobilize tagged proteins after five consecutive heat-cooling treatment (Hx, x = round of heat-cooling treatment). (B) NPP-S could immobilize tagged proteins after five consecutive freeze-thaw treatment (Fx, x = round of freeze-thaw treatment). NPP, SnoopCatcher-PhaC-SpyCatcher fusion protein; SnGFP-NPP-L, SnGFP-NPP ligated protein; NPP-SpBLA-L, NPP-SpBLA ligated protein; SnGFP-NPP-SpBLA-L, SnGFP-NPP-SpBLA ligated protein.