FIGURE 8.
Assessment of virulence of AB5075 mutant and complemented strains in a murine pneumonia model. Mice were infected intranasally with approximately 5 × 106 CFU of the indicated strain and survival was monitored for 5 days. Experiments with the cusR mutant strain and complemented derivative were performed in four biological replicates with five mice per group (n = 20 per strain), spaced two and two over time to ensure reproducibility; two biological replicates were completed with the copD mutant strain and complemented derivative (n = 10 mice per strain). Kaplan–Meier survival curves were compared using the Mantel–Cox test with Holm’s correction for multiple comparisons. Statistically significant comparisons are indicated by bars with asterisks signifying the P-value as follows: *P < 0.05; **P < 0.01; ***P < 0.001. The only significant comparison in the top panel is wild-type AB5075 vs. the cusR mutant strain.