Table 1.
The accuracy (intra- and inter-day), precision (intra- and inter-day), recovery, matrix effects, and stability for 16 compounds in mice (n = 6).
| Compounds | Accuracy (%) n = 6 |
Precision (RSD, %) n = 6 |
Recovery | Matrix Effect | Auto-sampler for 24 h | At −80 ℃ for 1 Month | Freeze–thaw Cycles | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Intra-day | Inter-day | Intra-day | Inter-day | Accuracy (%) |
Precision (%) |
Accuracy (%) |
Precision (%) |
Mean (%) |
RSD (%) |
Mean (%) |
RSD (%) |
Mean (%) |
RSD (%) |
|
| tauroursodeoxycholic acid | 95.36 | 98.09 | 7.56 | 6.27 | 89.19 | 8.83 | 99.09 | 6.29 | 99.32 | 5.44 | 99.51 | 3.03 | 100.74 | 5.48 |
| taurochenodeoxycholic acid | 90.42 | 92.78 | 12.76 | 4.34 | 97.78 | 3.18 | 103.23 | 3.41 | 101.22 | 10.67 | 103.19 | 3.52 | 99.57 | 6.97 |
| taurocholic acid | 97.63 | 98.99 | 7.90 | 5.97 | 92.23 | 3.13 | 102.50 | 5.31 | 100.82 | 4.95 | 100.15 | 9.39 | 98.27 | 5.45 |
| taurolithocholic acid | 102.82 | 91.61 | 11.59 | 4.63 | 99.97 | 6.02 | 88.79 | 6.54 | 103.31 | 10.97 | 99.89 | 9.92 | 99.52 | 5.62 |
| cholic acid | 95.84 | 101.32 | 7.50 | 7.32 | 92.23 | 9.84 | 101.34 | 10.65 | 98.83 | 3.28 | 97.98 | 10.47 | 97.59 | 6.75 |
| chenodeoxycholic acid | 87.40 | 89.53 | 4.84 | 9.81 | 103.30 | 7.78 | 93.22 | 7.74 | 95.82 | 9.19 | 96.16 | 3.92 | 97.92 | 6.84 |
| ursodeoxycholic acid | 90.07 | 98.47 | 11.99 | 4.48 | 99.97 | 8.10 | 90.18 | 10.16 | 96.85 | 9.22 | 98.24 | 8.31 | 98.09 | 5.44 |
| hyodeoxycholic acid | 104.10 | 94.66 | 6.06 | 12.57 | 86.89 | 5.07 | 100.11 | 7.20 | 100.43 | 4.92 | 99.92 | 3.95 | 98.48 | 4.79 |
| deoxycholic acid | 89.52 | 96.09 | 7.25 | 10.92 | 96.83 | 5.80 | 95.69 | 7.34 | 101.62 | 3.85 | 97.95 | 8.34 | 96.93 | 10.83 |
| beta-muricholic acid | 98.94 | 92.92 | 8.48 | 6.12 | 100.07 | 3.09 | 99.37 | 6.78 | 95.95 | 8.19 | 99.53 | 4.93 | 100.98 | 3.14 |
| lithocholic acid | 91.70 | 104.72 | 13.48 | 3.24 | 94.44 | 8.56 | 100.24 | 11.02 | 101.28 | 9.39 | 97.83 | 4.26 | 103.25 | 6.24 |
| glycochenodeoxycholic acid | 93.37 | 90.07 | 5.16 | 6.04 | 98.31 | 4.90 | 87.94 | 4.32 | 104.33 | 5.31 | 97.80 | 3.18 | 101.10 | 6.73 |
| glycoursodeoxycholic acid | 98.37 | 95.36 | 10.27 | 10.73 | 97.50 | 7.86 | 90.37 | 9.11 | 98.61 | 3.50 | 98.84 | 10.16 | 96.30 | 11.6 |
| glycocholic acid | 89.42 | 97.84 | 6.05 | 10.87 | 99.68 | 5.52 | 103.59 | 4.21 | 95.93 | 7.04 | 104.37 | 8.52 | 98.85 | 5.66 |
| glycohyodeoxycholic acid | 102.15 | 98.75 | 3.97 | 3.97 | 95.61 | 4.89 | 96.66 | 3.42 | 98.68 | 3.80 | 97.86 | 5.14 | 101.24 | 10.98 |
| glycolithocholic acid | 92.99 | 96.89 | 9.24 | 7.96 | 95.96 | 7.25 | 88.77 | 5.12 | 101.37 | 7.89 | 96.36 | 5.50 | 99.33 | 10.46 |