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. 2019 Apr 8;48(3):1112–1126. doi: 10.1007/s10439-019-02257-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Assembly of the OSPB chamber and final system. (a); (b) the construct was firmly fixed in the clamp using tweezers; (c) the two clamps located on both sides of the chamber were activated thanks to an external screw with a screwdriver; (d) the chamber was closed and filled with culture medium; (e) complete OSPB system consisting in six separate chambers mounted on the OPB oscillating platform and placed in a standard cell culture incubator.