Figure 5.

H&E and collagen type I and III immunostaining of samples, 7 days after static or dynamic culture. On the surfaces of constructs, the newly synthesized extracellular matrix appeared thinner, looser and less organized in the SC constructs, whereas in the DC group it was more dense and structured. The newly synthesized extracellular matrix is generally poor in type I collagen, but rich in type III collagen. DC constructs produced a more positive type I and type III collagen than that deposited by the SC group. Magnification ×200, scale bars 100 µm.