Fig. 2.

An exemplary blot and relative level of COX-2 (a), Nrf2 (b), PPARγ (c), and FABP4 (d) in RAW 264.7 cells supplemented with L. edodes mycelial extracts and activated with LPS. Vehicle—control cells; LPS—cells activated with lipopolysaccharide; L. edodes—cells incubated with extract from control mycelium; L. edodes+CuSO₄—cells incubated with extract enriched with CuSO4; L. edodes + CuGlu—cells incubated with extract enriched with C₁₂H₂₂CuO₁₄; L. edodes + ZnSO₄—cells incubated with extract enriched with ZnSO₄; L. edodes + ZnHasp—cells incubated with extract enriched with C₈H₁₂N₂O₈Zn; L. edodes + Se—cells incubated with extract enriched with Se(IV) 50 mg/L medium. Values are presented as means ± SD. Data are expressed as in relation to β-actin. *p < 0.001 relative to vehicle group, ^#p < 0.001 relative to LPS-activated cells