Table 1.
Content of elements (mg/100 g d.w.) determined in the fruiting bodies and mycelium, as well as in basic media and the ones enriched with Zn, Cu and Se used for in vitro culturing of L. edodes
| L. edodes | Metals | |||||||
|---|---|---|---|---|---|---|---|---|
| K | Ca | Mn | Fe | Cu | Zn | Se | Ga | |
| L. edodes (fruiting bodies) | 1839.5 ± 89.1a | 312.2 ± 56.2a | 2.9 ± 0.2a | 14.5 ± 2.6a | 1.6 ± 0.3a | 7.6 ± 0.4a | 0.01 ± 0.00 | 50.0 ± 0.0 |
| L. edodes (control mycelium) | 1035.3 ± 273.6a,b | 684.4 ± 127.1a,b | 18.9 ± 3.4a,b | 14.5 ± 2.5b | – | 17.2 ± 2.4b | 0.01 ± 0.00 | 50.0 ± 0.0 |
| L. edodes (control medium) | 1828.6 ± 7.5b,c | 93.4 ± 5.9a,b,c | 17.5 ± 0.1a,c | 12.9 ± 1.1c | – | 6.0 ± 0.1b,c | – | 50.0 ± 0.0 |
| L. edodes + ZnSO4 (mycelium) | 780.8 ± 51.5a,c,d | 234.5 ± 26.1b,c | 9.8 ± 0.8a,b,c,d | 9.7 ± 1.4a,b,d | 3.2 ± 0.1a,d | 231.2 ± 6.5a,b,c,d | 0.01 ± 0.00 | 50.0 ± 0.0 |
| L. edodes + ZnSO4 (medium) | 391.9 ± 17.4a,b,c,d | 123.4 ± 6.9a,b,e | – | 9.7 ± 1.3a,b,e | 14.3 ± 0.3a,d | 0.6 ± 0.3b,d,e | 0.01 ± 0.00 | 50.0 ± 0.0 |
| L. edodes + ZnHasp (mycelium) | 1069.4 ± 257.4a,c,d | 295.7 ± 58.6b,c,e | 13.9 ± 1.3a,b,c,d | 13.2 ± 3.9f | – | 141.2 ± 13.8a,b,c,d,e,f | 0.01 ± 0.00 | 50.0 ± 0.0 |
| L. edodes + ZnHasp (medium) | 5103.9 ± 131.6a,b,c,d | 215.6 ± 55.2b,c | 13.5 ± 0.2a,b,c,d | 23.1 ± 0.5a,b,c,d,e,f | – | 257.3 ± 0.7a,b,c,d,e,f | – | 50.0 ± 0.0 |
| L. edodes + CuSO4 (mycelium) | 1002.5 ± 81.8a,c,d | 362.3 ± 52.1b,c,d | 15.1 ± 0.8a,b,d | 11.9 ± 2.6d | 21.0 ± 1.8a | 11.1 ± 1.7a,b,c,d | 0.5 ± 0.2a,b | 50.0 ± 0.0 |
| L. edodes + CuSO4 (medium) | 2650.7 ± 132.6a,d,e | 35.1 ± 5.0a,b,d,e | 19.5 ± 0.1a,d,e | 21.7 ± 0.6a,b,c,d,e | 97.0 ± 1.0a,d,e | – | 0.4 ± 0.0a,b,e | 50.0 ± 0.0 |
| L. edodes + CuGlu (mycelium) | 1084.0 ± 103.6a,c,e,f | 396.3 ± 118.8b,c | 15.4 ± 0.8a,b,e | 14.6 ± 4.4e,f | 20.4 ± 2.3a,e,f | 9.4 ± 1.5b,c,f | 0.01 ± 0.0d | 50.0 ± 0.0 |
| L. edodes + CuGlu (medium) | 391.9 ± 17.4a,b,c,d | 123.4 ± 6.9a,b,d | – | 9.7 ± 1.3a,b,e,f | 14.3 ± 0.4a,d,e | 0.6 ± 0.3a,b,c,d,f | – | 50.0 ± 0.0 |
| L. edodes + Se(IV) 25 mg/L medium (mycelium) | 803.6 ± 16.5a,c,d | 420.1 ± 39.6a,b,c | 18.7 ± 1.0a,d | 2.71 ± 1.5a,b,c,d | – | 18.5 ± 1.7a,c,d | 114.3 ± 15.3a,b,c | 50.0 ± 0.0 |
| L. edodes + Se(IV) 25 mg/L medium (medium) | 2602.8 ± 155.2a,b,c | 57.1 ± 4.4a,b,d,e | 16.5 ± 0.8a | 8.4 ± 0.1a,b,e,d,f | 28.8 ± 0.2a,b,e | 23.6 ± 1.7a,b,c,d,e | 40.3 ± 3.3b,c,d,e | 50.0 ± 0.0 |
| L. edodes + Se(IV) 50 mg/L medium (mycelium) | 621.8 ± 23.0a,b,c,e,f | 359.7 ± 62.3b,c,e | 14.9 ± 1.3a,b,d | 13.5 ± 1.9d,e,f | 7.9 ± 0.5e,f | 15.1 ± 1.6a,c,d,e,f | 568.6 ± 62.5a,b,c,d | 50.0 ± 0.0 |
| L. edodes + Se(IV) 50 mg/L medium (medium) | 2599.7 ± 149.2a,b,c,d | 56.18 ± 4.2a,b,d | 17.3 ± 1.0.a | 7.3 ± 0.8a,b,c,d,f | 24.0 ± 1.2a,b,f | 27.1 ± 1.3a,b,c,d,e | 112 ± 15.1a,b,c,d | 50.0 ± 0.0 |
Data are presented as mean ± standard deviation; n = 6 repetitions. Gallium was used as an internal standard. Tukey–Kramer test was used to reveal the differences between paired groups of elements in rows compared to fruiting bodies, control mycelium, and control medium; the same letters (a, b, c, d, e, and f) are marked for which the content differences are statistically significant (for p values < 0.05) (GraphPad InStat)