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The human milk oligosaccharides 2'-FL inhibits necroptosis in tissue explants from premature infants. (A–D) qRT-PCR of inflammation (IL6) or necroptosis (Ripk1, Ripk3, Mlkl) genes in tissue from premature infants exposed to either control or NEC bacteria, in the presence or absence of 2'-FL. Each data point is a technical replicate from a tissue donor. *P < .05, **P < .01, and ∗∗∗P < .001. Ctrl bact, human control bacteria; NEC bact, human NEC bacteria.
