Skip to main content
. 2019 Nov 19;9(3):403–423. doi: 10.1016/j.jcmgh.2019.11.002

Figure 2.

Figure 2

An experimental mouse model of NEC shows necroptosis activation biased toward intestinal villus tips. (A–D) qRT-PCR of necroptosis or TNFα expression in mouse ileum. (E–G) Western blot and protein quantification from control and NEC mouse ileum. (F and G) Each data point represents a separate animal. β-actin was used as the loading control. (I–K) IHC of the intestinal ileum from mice at p10. Cells near the tips of the intestinal villi show positive staining for pRIPK3 in the NEC model (arrows in J). (L) Quantification of pRIPK3 IHC from NEC animals along the villi from the villus tip toward the crypt base. Dark red cells on the illustration represent pRIPK3-positive cells. The center line on the graph represents the mean fluorescence signal, and the shaded area shows the SD from multiple villi scanned. n = 5 animals analyzed. Scale bars: 10 μm. *P < .05 and ***P < .005. Each dot represents a separate patient as indicated; red circles and green circles reflect the same respective sample group cluster. a.u., arbitrary units; Ctrl, control; DAPI, 4′,6-diamidino-2-phenylindole; MW, molecular weight; Ripk3 KO, knockout animals.