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. 2019 Nov 19;9(3):403–423. doi: 10.1016/j.jcmgh.2019.11.002

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

The formula feeds, hypoxia, and bacteria in combination are required for the induction of necroptosis genes in mouse ileum with NEC. (A–C) qRT-PCR of the indicated gene in 10-day-old C57-BL/6 mouse ileum exposed to either breast feeds (controls) or the NEC model with a combination of formula, bacteria, and/or hypoxia alone or in combination as indicated. Each data point is a separate mouse. Three separate experiments were performed. **P < .01 and ***P < .001 as indicated. Ctrl, control.