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. 2019 Nov 19;9(3):403–423. doi: 10.1016/j.jcmgh.2019.11.002

Figure 4.

Figure 4

NEC causes an increase in reactive oxygen species and inflammatory cytokines in the intestinal epithelium that is abrogated through genetic or pharmacologic inhibition of necroptosis. (A–D) Reactive oxygen species staining shown using an antibody against 3NT in control, NEC, and necroptosis-inhibited NEC (Mlkl knock out [KO] and Nec1s). (E) Quantification and (F) distribution of the 3-NT signal. (E) Each data point represents an individual animal. (F) Compiled from NEC animals only. The center line represents the mean, and the shaded area shows the SD. n = 5. (G) qRT-PCR of inducible nitric oxide synthase (iNos) in control or NEC animals with indicated treatments. Scale bar: 10 μm. *P < .05, and ***P < .0001. a.u., arbitrary units; Ctrl, control; DAPI, 4′,6-diamidino-2-phenylindole; Ecad, E-cadherin; iNOS, inducible nitric oxide synthase.