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. 2019 Nov 19;9(3):403–423. doi: 10.1016/j.jcmgh.2019.11.002

Figure 8.

Figure 8

Inhibition of necroptosis, or addition of human milk oligosaccharides or human breast milk protects against necroptosis and tissue damage in an ex vivo NEC model (NEC-in-a-dish). (A) Confocal micrographs of undifferentiated or differentiated enteroids grown in the presence or absence of hypoxia and NEC bacteria (Nec-in-a-dish). Tissue architecture (ie, E-cadherin), proliferation (ie, PCNA), and necroptosis activation (ie, pRIPK3) staining are shown. (B) Immunohistochemical quantification of necroptosis (pRIPK3) from panel A. (C) Immunohistochemical quantification of proliferation (PCNA) from panel A. Differentiation was for 36 hours. Each dot is a separate sample. Representative of 3 separate experiments. Scale bar: 10 μm. *P < .05, *P < .005, and ***P < .0005. a.u., arbitrary unit; ctrl, control; DAPI, 4′,6-diamidino-2-phenylindole; Ecad, E-cadherin.