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. 2019 Nov 19;9(3):403–423. doi: 10.1016/j.jcmgh.2019.11.002

Figure 9.

Figure 9

Bacteria or hypoxia-only controls for NEC-in-a-dish experiments. (A) Confocal micrographs of undifferentiated or differentiated enteroids grown in the presence or absence of NEC bacteria. (B) The same experiment as in panel A, but with hypoxia instead of bacteria. Tissue architecture is represented by E-cadherin, proliferation by PCNA, and necroptosis activation by pRIPK3. Differentiation was for 36 hours. Scale bar: 10 μm. Representative of 3 separate experiments. DAPI, 4′,6-diamidino-2-phenylindole; Ecad, E-cadherin.