Table 1.
Primers
| Gene | Forward primer | Reverse primer | Amplicon size, bp |
|---|---|---|---|
| Mouse primers | |||
| Ascl2 | ATGGAGCAGGAGCTGCTTGACTTT | TTCTTGGGCTAGAAGCAGGTAGGT | 110 |
| ChgA | AAGGTGATGAAGTGCGTCCTGGAA | AGCAGATTCTGGTGTCGCAGGATA | 137 |
| Hopx | TTCAACAAGGTCAACAAGCACCCG | CCAGGCGCTGCTTAAACCATTTCT | 106 |
| IL6 | CCAATTTCCAATGCTCTCCT | ACCACAGTGAGGAATGTCCA | 182 |
| IL1β | AGTGTGGATCCCAAGCAATACCCA | TGTCCTGACCACTGTTGTTTCCCA | 175 |
| iNOS | CTGCTGGTGGTGACAAGCACATTT | ATGTCATGAGCAAAGGCGCAGAAC | 167 |
| Ki67 | CCAAGGCCCAAGTTTGATGC | GACTTGGCCCCGAGATGTAG | 138 |
| Lgr5 | TGAGCGGGACCTTGAAGATTTCCT | TACCAAATAGGTGCTCACAGGGCT | 116 |
| Lyz1 | AAGCTGGCTGACTGGGTGTGTTTA | CACTGCAATTGATCCCACAGGCAT | 178 |
| Mlkl | CAAACAGTGAAGCCCCCTGA | GTATAAGCCTCTGGCTGCCC | 141 |
| Muc2 | TAGTGGAGATTGTGCCGCTGAAGT | AGAGCCCATCGAAGGTGACAAAGT | 168 |
| Ripk1 | TTGTGCCTTTAGGAAGCCCA | GTGCAGCCCATCCTAACAGT | 108 |
| Ripk3 | GCAAGGAGTCAGGGGAATCA | TTTGTAGTCTTTGACCTCTTGTTG | 145 |
| Sis (SI) | ATCCAGGTTCGAAGGAGAAGCACT | TTCGCTTGAATGCTGTGTGTTCCG | 154 |
| Tnfα | TTCCGAATTCACTGGAGCCTCGAA | TGCACCTCAGGGAAGAATCTGGAA | 144 |
| Human primers | |||
| Mlkl | CGGCCCTCTGTGGATGAAAT | TGTCCAGTTTGTGCCTCTCC | 113 |
| Ripk1 | CCGGTCAAATTCAGCCACAG | CTGTTTCGTCTGCCTGTCCA | 199 |
| Ripk3 | CCCAGACTCCAGAGACCTCA | GGTCGCCCTGTTACTGGATT | 157 |
| Tnfα | GGCGTGGAGCTGAGAGATAAC | GGTGTGGGTGAGGAGCACAT | 120 |
| Genotyping primers | |||
| Mlkl k/o |
TATGACCATGGCAACTCACG, ACCATCTCCCCAAACTGTGA TCCTTCCAGCACCTCGTAAT (wild type, 498 bp; knockout, 158 bp) |
||
| Ripk3 k/o |
CGCTTTAGAAGCCTTCAGGTTGAC, GCAGGCTCTGGTGACAAGATTCATGG CCAGAGGCCACTTGTGTAGCG (wild type, 780 bp; knockout, 485 bp) |
||
| Tlr4 k/o |
CTTCAAGGATCCGATGATGAG, TGCTTTGCTTAACCCAGTGA (knockout, 328 bp) | ||
k/o, knockout.