Fig. 1.
Expression of Reg3β and infiltration of RORγt+ cells in the small intestine of CFLARs Tg mice at the embryonic stage. (A, B) Small intestine sections from wild-type (WT), XCFY, and XCFX mice on a Rorc-gfp/+ genetic background at E18.5 were stained with anti-GFP (green) and anti-Reg3β (red) (A), or anti-pSTAT3 antibodies (magenta) (B). Lower panels are enlarged images of white boxes in the upper panels (A). White arrowheads indicate RORγt+ cells. Scale bars, 100 μm. (C) Expression of Reg3β in the small intestine and colon of adult wild-type mice. Tissue extracts were prepared from the small intestine and colon of 8- to 12-week-old wild-type mice and examined by Western blotting with the indicated antibodies. Each number indicates an individual mouse. Results represent two independent experiments. (D) mRNA was prepared from the small intestine and colon of 8- to 12-week-old mice, and the expression of Reg3b and Reg3g was determined by qPCR. Results are mean ± SEM (n = 12 mice). Statistical significance was determined using the two-tailed unpaired Student's t-test. ***P < 0.001.