Fig. 3.

Generation of Reg3g^−/− mice. (A) Deletion of exon 3 of Reg3g genes in two lines of Reg3g^−/− mice. Red characters indicate deleted nucleotides and mutated amino acids. (B) Tissue extracts were prepared from the small intestine of 8- to 12-week-old Reg3g^+/+ and Reg3g^−/− mice (line G151) and examined by Western blotting with the indicated antibodies. Each number indicates an individual mouse. Results represent two independent experiments. (C) In-frame deletion of exon 3 of Reg3b in two lines of Reg3g^−/− mice. Red characters indicate the deleted nucleotides. (D) Paraffin-embedded tissue sections of the small intestine and colon of 8- to 12-week-old Reg3g^+/+ and Reg3g^−/− mice (line G151) were stained with H&E. Scale bars, 100 μm.