Figure 3.

LincRNA00494 mediated proliferation of the NSCLC cells. (A) FISH showed that LincRNA00494 was mostly located in the cytoplasm, with a small portion in the nucleus. Green indicates vimentin; red indicates LincRNA00494. (B) Cells were harvested, and the RNA stability of LincRNA00494 was analyzed by RT-qPCR relative to time 0 after new RNA synthesis was blocked with actinomycin D; the data are shown as the mean ± SEM (normalized to GAPDH). (C) H358 and H1299 cells were seeded in 96-well plates after transfection, and the cell proliferation was assessed daily for 5 days using the CCK-8 assay. Six replicates were performed for each group, and the experiment was repeated three times. Data are shown as the mean ± SEM. (D,E) The data show the tumor volumes of the xenografts in each group 4 weeks after subcutaneously implantation of stable NSCLC cells. The mean tumor volumes from six nude mice from each group are shown at different time points.