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. 2020 Jan 22;23:37–45. doi: 10.1016/j.jare.2020.01.009

Fig. 1.

Fig. 1

aSyn-overexpression in SCNA-gene transfected SH-SY5Y neurons. (a) Representative Western blots of positively SCNA gene transfected clones (lanes 3 and 4, low-aSyn; lanes 5 and 6, high-aSyn) compared to the empty plasmid-transfected clone (lanes 1 and 2, Plasmid). (b) aSyn expression was quantified by optical densitometry, then normalized with loading (β-actin) controls, and finally represented like the percentage of the plasmid value (100%): Values are expressed as the mean ± SD of 5 independent experiments with Bonferroni post hoc tests: **P < 0.01, ***P < 0.0001 vs. Plasmid; +++P < 0.0001 vs. low-aSyn.