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Viability of aSyn-overexpressing SH-SY5Y neurons during subculturing. Moderate levels of aSyn overexpression provoked cytotoxicity at low passage, but not at high passage. High-aSyn overexpression increased cell viability regardless the passage. Values are expressed as the mean ± SD of 24 independent experiments with Bonferroni post hoc tests: ***P < 0.0001 vs. Plasmid; +++P < 0.0001 vs. low-passage aSyn counterparts.
