Fig. 4.
Colony formation in aSyn-overexpressing SH-SY5Y neurons. Colony counting in (a) attachment-dependent (clonogenicity) conditions and (b) attachment-independent (tumorigenicity) conditions. Cm: conditioned medium from high-aSyn culture. Data represent the mean ± SD of 3 (clonogenicity) to 4 (tumorigenicity) different experiments with Bonferroni post hoc test ***P < 0.001 compared to Plasmid; ++P < 0.01, +++P < 0.001 compared to low-aSyn. (c) Images of crystal violet-stained colonies grown from different clones as observed in agar plates.