Fig. 5.

Rotenone-induced neurotoxicity in aSyn overexpressing neurons and worsening effects of PRGO. (a) Cytotoxity of rotetone (Rot) in SH-SY5Y neurons. The 24-hour treatment with Rot damaged only aSyn-overexpressing neurons. (b) Pretreatment with 50 µg/mL of partially reduced graphene oxide (PRGO) triggers Rot cytotoxicity in plasmid-transfected cells while worsening the conditions for low-aSyn cells. Grey bars represent the viability (100%) in vehicle control. Cell viability was calculated as the percentage of vehicle control of each clone type. Data represent the mean ± SD of 8 independent experiments with Bonferroni post hoc tests: ***P < 0.001 compared to Plasmid; ⁺⁺⁺P < 0.001 compared to low-aSyn.