Figure 3.

Confirmation of Subcellular Localization of circFNDC3B

(A) qRT-PCR for the abundance of circFNDC3B and FNDC3B in TPC-1 cells treated with actinomycin D at the indicated time point. The error bars represent SD (n = 3). (B) qRT-PCR for the expression of circFNDC3B and FNDC3B mRNA in TPC-1 cells treated with or without RNase R. The results indicated that circFNDC3B was resistant to RNase R digestion. (C) Levels of circFNDC3B in the nuclear and cytoplasmic fractions of TPC-1 cells. The results showed that circFNDC3B was predominantly localized in the cytoplasm. (D) The CCK8 assay showed that circFNDC3B knockdown significantly repressed cell proliferation of TPC-1 cells. (E) The CCK8 assay showing overexpression of circFNDC3B promoted the proliferation of K1 cells. (F) Colony-formation assay showed that the knockdown of circFNDC3B significantly restrained the proliferation of TPC-1 cells. (G) Colony-formation assay showed that the ectopic expression of circFNDC3B significantly promoted the proliferation of K1 cells. (H) The flow cytometry analysis showed that circFNDC3B knockdown led to an arrest in the G1 phase of TPC-1 cells. (I) The flow cytometry analysis showed that overexpression of circFNDC3B decreased the G0/G1-phase percentage of K1 cells. Data are listed as mean ± SD of at least three independent experiments. **p < 0.01.