Figure 5.

Stability of the Obtained Aptamer, Apt01 and Apt02, during Incubation in Medium 200PRF with LSGS Kit for 24 h, as Determined by Denaturing Urea Polyacrylamide Gel Electrophoresis (PAGE)

(A) Representative images of denaturing urea PAGE. Bands of intact aptamers are indicated with arrows. (B) Graphical representation of PAGE results. The fraction of intact aptamers (relative to the sample without incubation in Medium 200 with LSGS kit) was plotted as a function of time. Stained DNA was imaged with Digi-Gel Shot (Takara Bio) and quantified using ImageJ software package. Almost Apt01 was degraded in cell culture medium even at 4 h, whereas even at 24 h, 86% of Apt02 remained in the culture medium. The bar graphs represent means ± standard error for three independent experiments.