Figure 6.

In Vitro Tube Formation Assay Using Human Umbilical Vein Endothelial Cells (HUVECs) on a Three-Dimensional Gel Consisting of Diluted Matrigel

The cells were treated with Apt01 (10 μM), Apt02 (10 μM), or VEGF165 (10 ng/mL, 0.26 nM) for 24 h. (A) Representative images of tube formation of HUVECs on Matrigel, which are treated by aptamers or VEGF165. Scale bars, 1 mm. (B) Time course of mesh number in the images of the HUVEC networks at 4, 7, and 24 h. Error bars represent standard deviation of the mean (n = 3 plots). (C–E) Boxplot showing mesh number in the images of the HUVEC networks at (C) 4, (D) 7, and (E) 24 h (n = 3 plots). *p < 0.05 as compared with control; **p < 0.05 as compared with Apt01.