Figure 1.

Deregulated circRNAs in PTC Tumor Tissues

(A) The heatmap showed the top 10 most increased and decreased circRNAs in PTC tissues as compared to that in the matched non-tumor tissues analyzed by circRNA ArrayStar chip. (B) The level of circRASSF2 was significantly increased in tumor tissues as compared to that in matched non-tumor tissues of 112 pairs of PTC patients. ***p < 0.001. (C) The level of circRASSF2 in PTC patients with metastasis was significantly higher than that in patients without metastasis. ***p < 0.001. (D) The levels of circRASSF2 in PTC cells, compared to that in a human normal thyroid epithelium cell Nthy-ori 3-1. (E) qRT-PCR for the abundance of circRASSF2 and RASSF2 in PTC cells treated with actinomycin D at the indicated time point. (F) circRASSF2 was resistant to RNase R digestion in PTC cells. **p < 0.01. (G) Levels of circRASSF2 in the nuclear and cytoplasmic fractions of PTC cells. **p < 0.01. (H) Levels of circRASSF2 in the nuclear and cytoplasmic fractions of K1 cells. **p < 0.01. Data are the means ± SD of three experiments.