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. 2020 Jan 21;23:25–35. doi: 10.1016/j.jare.2020.01.006

Fig. 5.

Fig. 5

TMEM16A inhibition blocked cerulein-induced NFκB activation in AR42J cells. A. Representative Western blot results (Top) of p65 expression in the nucleus and cytosol in control AR42J cells and cerulein-treated cells in the presence or absence of BAPTA-AM (13 μM). DMSO was used as vehicle control. Bottom: quantification results of the ratio of nuclear/cytosolic p65 expression. n = 3. *p < 0.05 vs control; #p < 0.05 vs DMSO. B. Western blot results (Top) of p65 expression in the nucleus and cytosol in control AR42J cells and cerulein-treated cells with or without transfection of scrambled shRNAs (Scr) or TMEM16A-shRNAs. Bottom: quantification results of the ratio of nuclear/cytosolic p65 expression. n = 3. *p < 0.05 vs control; #p < 0.05 vs scrambled shRNAs. C. ELISA results of the IL-6 levels in the culture medium in control AR42J cells and cerulein-treated cells with or without transfection of scrambled shRNAs or TMEM16A-shRNAs. n = 6. *p < 0.05 vs control; #p < 0.05 vs scrambled shRNAs.