Effects of ketamine pretreatment on BDNF-TrkB signaling in selected tissues. (A) mBDNF [Group: F
(1,
28) = 15.66, P < 0.01; Ketamine: F
(1,
28) = 5.762, P < 0.05; interaction: F
(1,
28) = 14.18, P < 0.001], p-TrkB/TrkB [Group: F
(1,
28) = 29.8, P < 0.001; Ketamine: F
(1,
28) = 4.507, P < 0.05; interaction: F
(1,
28) = 10.55, P < 0.01] and TrkB [Group: F
(1,
28) = 0.1302, P = 0.7209; Ketamine: F
(1,
28) = 0.1722, P = 0.6813; interaction: F
(1,
28) = 0.0913, P = 0.7648] levels in mPFC. (B) mBDNF [Group: F
(1,
28) = 3.217, P = 0.0837; Ketamine: F
(1,
28) = 6.182, P < 0.05; interaction: F
(1,
28) = 9.922, P < 0.01], p-TrkB/TrkB [Group: F
(1,
28) = 8.677, P < 0.01; Ketamine: F
(1,
28) = 18.61, P < 0.001; interaction: F
(1,
28) = 5.491, P = 0.0265] and TrkB [Group: F
(1,
28) = 0.1697, P = 0.7209; Ketamine: F
(1,
28) = 0.1697, P = 0.6835; interaction: F
(1,
28) = 0.0787, P = 0.7812] levels in the hippocampus. (C) mBDNF [Group: F
(1,
28) = 6.854, P < 0.05; Ketamine: F
(1,
28) = 1.919, P = 0.1769; interaction: F
(1,
28) = 9.254, P < 0.01], p-TrkB/TrkB [Group: F
(1,
28) = 1.571, P = 0.2204; Ketamine: F
(1,
28) = 0.0181, P = 0.8938; interaction: F
(1,
28) = 0.1937, P = 0.6632] and TrkB [Group: F
(1,
28) = 2.286, P = 0.1417; Ketamine: F
(1,
28) = 5.919, P < 0.05; interaction: F
(1,
28) = 6.185, P < 0.05] levels in the liver. (D) mBDNF [Group: F
(1,
28) = 8.803, P < 0.01; Ketamine: F
(1,
28) = 8.419, P < 0.01; interaction: F
(1,
28) = 2.964, P = 0.0962], p-TrkB/TrkB [Group: F
(1,
28) = 1.003, P = 0.3251; Ketamine: F
(1,
28) = 1.134, P = 0.1961; interaction: F
(1,
28) = 0.0105, P = 0.9192] and TrkB [Group: F
(1,
28) = 3.494, P = 0.0763; Ketamine: F
(1,
28) = 20.16, P = 0.0002; interaction: F
(1,
28) = 7.58, P = 0.0123] levels in the muscle. Data are shown as mean ± SEM (n = 8). *P < 0.05, **P < 0.01, or ***P < 0.001. mBDNF, mature brain derived neurotrophic factor; mPFC, medial prefrontal cortex; N.S., not significant; POD, postoperative depression; p-TrkB, phosphorylated tropomyosin-related kinase B; TrkB, tropomyosin-related kinase B.