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. 2020 Feb 6;10:1702. doi: 10.3389/fphar.2019.01702

Figure 7.

Figure 7

ANA-12 attenuated ketamine-induced beneficial effects on POD-like symptoms in depression susceptible mice. (A) The schedule of treatment and behavioral tests. OFT and TST were performed on day 1 and 8 after A+S, respectively; FST was performed on day 2 and 9 after A+S, respectively; SPT was performed on days 6 and 13 after A+S, respectively. Vehicle or saline (10 ml/kg), Ketamine (10 mg/kg), or ANA-12 (0.5 mg/kg) was intraperitoneally injected at a single dose on day 8. (B) Dendrogram of hierarchical clustering analysis. A total of 44 mice after A+S were divided into depression susceptible, resilient, and undetermined groups by TST, FST, and SPT results of hierarchical clustering analysis. (C) OFT [time: F (1, 20) = 0.1232, P = 0.7293; group: F (3, 20) = 0.0042, P = 0.9996; interaction: F (3, 20) = 0.09238, P = 0.9634]. (D) TST [time: F (1, 20) = 0.0211, P = 0.8859; group: F (3, 20) = 3.35, P = 0.0396; interaction: F (3, 20) = 17.93, P < 0.0001]. (E) FST [time: F (1, 20) = 1.67, P = 0.211; group: F (3, 20) = 1.878, P = 0.1659; interaction: F (3, 20) = 1.16, P = 0.3497]. (F) SPT [time: F (1, 20) = 1.856, P = 0.1882; group: F (3, 20) = 0.7368, P = 0.5424; interaction: F (3, 20) = 3.35, P = 0.0396]. Data are shown as mean ± S.E.M. (n = 6 per group). **P < 0.01 A+S: anesthesia and surgery; ANA-12: N-[2-[[(Hexahydro-2-oxo-1H-azepin-3-yl)amino]carbonyl] phenyl]-benzo[b] thiophene-2-carboxamide; FST, forced swimming test; N.S., not significant; OFT, open field test; POD, postoperative depression; SPT, sucrose preference test; TST, tail suspension test.