. 2019 Sep 19;57(2):775–786. doi: 10.1007/s13197-019-04110-z

Table 1.

Analysis of variance for the CCD models for sequential extraction and coefficients for the predictive models of the inulin and protein extraction/co-extraction yields. ANOVA was determined with a 95% confidence level

		S1^a									S2^a
		Protein extraction						Inulin extraction			Inulin extraction
		Protein yield			Inulin co-extraction yield			Inulin yield			Inulin yield			Protein co-extraction yield
Source	D.f.^b	Co-efficient	Sum of squares	P-value	Co-efficient	Sum of squares	P-value	Co-efficient	Sum of squares	P-value	Co-efficient	Sum of squares	P-value	Co-efficient	Sum of squares	P-value
Linear
Temp (T)	1	0.01	31.14	0.019	0.35	4.24	0.127	4.44	50.96	0.009	5.11	63.25	0.004	5.35	132.41	0.005
pH (P)	1	10.34	16.51	0.051	− 2	6.67	0.086	3.65	25.08	0.03	3.25	33.95	0.014	− 24	2.38	0.51
Quadratic
T²	1	0. 9	3.56	0.271	− 0.002	22.35	0.014	− 0.33	63.66	0.006	− 0.04	87.05	0.002	0.05	172.09	0.003
P²	1	− 0.94	98.64	0.002	− 0.33	11.89	0.039	3.99	12.66	0.077	4.05	7.315	0.13	0.599	40.03	0.04
Interaction
TP	1	− 0.004	0.19	0.78	− 0.02	2.89	0.21	0.04	0.125	0.82	0.1	1	0.517	− 0.96	92.16	0.01
Regression	1		150.06	0.001		48.59	0.01		151.3	0.01		439.06	0.01		192.39	0.01
Residual error	1		32.78			11.81			24.4			20.98			32.79
Lack of fit	3		24.05	0.12		6.61	0.3		16.32	0.208		27.97	0.084		24.05	0.09
Pure error	4		8.73			5.21			9.06			7.96			8.73
Model	12		189.59			65.33			171.28			236.56			440.7
R²		0.82			0.81			0.85			0.95			0.82
Adjusted R²		0.7			0.69			0.74			0.92			0.7

^aS1 and S2 is scenario 1 (protein extraction and inulin extraction first and second, respectively) and scenario 2 (inulin extraction first) for the sequential extraction, respectively

^bd.f. is the degrees of freedom