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. 2020 Feb 12;10:2494. doi: 10.1038/s41598-020-59225-7

Figure 8.

Figure 8

Long-term treatment for 72 h of human cardiac progenitor cells (hCPCs) with anti-aggregant dose (3.75 μM) of 2-oxo-clopidogrel (2-oxo-C) (A; in the presence of ADP 10 μM) does not recapitulate the ticagrelor-driven effects on release of TSG101 + (48KDa MW), CD63 + (52KDa MW) and heat shock protein (HSP)-70 + (70KDa MW) exosomes from hCPCs in the absence (B–D) or presence (E–G) of adenosine (ADE 10 μM) + EHNA (10 μM). Representative images of cropped densitometric bands of proteins TSG101, CD63, HSP70 are showed in each corresponding panel. The full-length blots/gels are presented in Supplemental Fig. 9 panel A. In addition, 2-oxo-C does not increase intracellular levels of phosphorylated histone H3 on Serine 10 (phospho-H3S10; 17KDa MW)/total histone H3 (H3; 17KDa MW) ratio in the absence (H) or presence (I) of adenosine (ADE 10 μM) + EHNA (10 μM). Representative images of cropped densitometric bands of proteins phospho-H3S10, H3 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are showed in each corresponding panel. The full-length blots/gels are presented in Supplemental Fig. 9 panel B. Levels of each analyzed protein are normalized on GAPDH (37KDa MW) levels and ratios are expressed as arbitrary units (a.u.). All measurements are mean ± SD. *p < 0.05 vs. control condition.