Figure 9.

Long-term pre-treatment for 48 h of normoxic HL1 cardiomyocytes with exosomes (100 μg) derived from hCPCs treated with anti-aggregant dose (3.75 μM) of 2-oxo-clopidogrel (2-oxo-C) does not prevent rising of cleaved caspase 3 (19KDa MW) and hypoxia inducible factor 1-alpha (HIF1-alpha; 110KDa MW) during severe hypoxia (1% O₂) in the absence (A,B) or presence (C,D) of adenosine (ADE 10 μM) + EHNA (10 μM). Representative images of cropped densitometric bands of proteins cleaved caspase 3, HIF1-alpha and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) are showed in each corresponding panel. The full-length blots/gels of abovementioned proteins in absence or presence of adenosine (ADE 10 μM) + EHNA (10 μM) are presented in panel A and B of Supplemental Fig. 10 respectively. Levels of each analyzed protein are normalized on GAPDH (37KDa MW) levels and ratios are expressed as arbitrary units (a.u.). All measurements are mean ± SD. *p < 0.05 vs. untreated condition (Vehicle: sterile phosphate buffer solution).