FIGURE 4.

Transcription of trfA correspond to the high protein levels and solubility of Spx. RNAs were purified from cells described in Figures 3B,C. The level of trfA RNA was measured by RTqPCR in non-treated cells and in cells treated for 30 min with either heat shock or oxidative stress induced by diamide. 16S RNA was used as a reference normalizing housekeeping gene. Fold change of trfA gene expression in treated samples relative to non-treated samples was normalized to a reference gene (16S RNA). The relative gene expression in non-treated cells was set to 1. (A) The levels of trfA were increased in the yjbH deletion mutant, where the levels of Spx were increased. (B) Transcription of trfA is decreased after heat shock and increased after oxidative stress, that correspond to solubility of Spx.