Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 12;10:2491. doi: 10.1038/s41598-020-59268-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of different coating substrates on dendrites of wild-type mouse hippocampal neurons. (A) DIV 12 mouse hippocampal neurons immunostained for dendrites cytoskeleton (MAP2 red) and nuclei (Hoechst blue) at different seeding cell densities with different coatings, such as Matrigel and poly-L-Ornithine (top lane) or only poly-L-Ornithine (bottom lane) (Scale bar = 100 µm). (B) Quantitative data on the number of neurons per mm2 counted at the different seeding cell densities. Data are expressed as mean ± SEM, n = 3 cultures for each condition. (C) Number of neurons (%) normalized on the number of counted viable nuclei (total cells), (D) average TDL per neuron (µm), (E) average number of dendrites endpoints per neuron and (F) average neuronal soma area (µm²). Number of neurons measured ranged from 1000 for the highest concentration seeded to 200 neurons for the lowest concentration seeded. Unpaired t-test to compare the different coating substrates at same concentration, *P ≤ 0.05; One-way ANOVA to compare TDL, endpoints and soma area at different dilutions (D–F).