Figure 3.

Upregulation of SNHG16 Is a Target of let-7c-5p in MG

(A) SNHG16 expression was examined in 24 MG patients and 29 control subjects by real-time PCR. (B) Transfection efficiency of let-7c-5p mimics was measured by real-time PCR. (C) The relative expression level of SNHG16 in Jurkat cells transfected with miRNA NC or let-7c-5p mimics was measured using real-time PCR. (D) The putative let-7c-5p binding sequence of the wild-type and mutation sequence of SNHG16. (E) The luciferase reporter plasmid containing SNHG16-WT or SNHG16-MUT was co-transfected with let-7c-5p mimics or miRNA NC into HEK293T cells. Luciferase activities were calculated as the ratio of firefly/Renilla activities. The experiment was repeated at least three times, and data are presented as the mean ± SD. **p < 0.01.