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. 2020 Jan 14;19:1027–1042. doi: 10.1016/j.omtn.2019.12.037

Figure 3.

Figure 3

The TCONS_00016233 Was Induced by LPS

HK-2 cells were treated with 50 μg/mL LPS, with or without TLR4 siRNA, p38MAPK agonist, or inhibitor. (A) RNA-FISH detection of intracellular localization of TCONS_00016233 in HK-2 cells. (B) qRT-PCR analysis of the expression levels of TCONS_00016233. (C) Immunoblot of phospho-p38 (p-p38)MAPK, p38MAPK, TLR4, and GAPDH. (D) Densitometric analysis of immunoblot bands. (E) qRT-PCR analysis of the expression levels of TCONS_00016233. (F) Immunoblot of p-p38MAPK, p38MAPK, and GAPDH. (G) Densitometric analysis of immunoblot bands. (H) qRT-PCR analysis of the expression levels of TCONS_00016233. (I) Immunoblot of p-p38MAPK, p38MAPK, TLR4, and GAPDH. (J) Densitometric analysis of immunoblot bands. (K) qRT-PCR analysis of the expression levels of TCONS_00016233. Data are expressed as mean ± SD (n = 6). #p < 0.05, LPS at 12 h and 24 h groups versus control group. *p < 0.05, LPS/TLR4 siRNA, p38MAPK inhibitor/LPS, or LPS/TLR siRNA/p38MAPK agonist versus LPS/scramble group. Δp > 0.05, LPS/TLR siRNA/p38MAPK agonist versus LPS/scramble group.