Figure 4.

LPS-Induced HK-2 Cell Apoptosis Was Attenuated by siRNA of TCONS_00016233

HK-2 cells were transfected with 50 nM TCONS_00016233 siRNA or scramble and then treated with or without 50 μg/mL LPS for 24 h. (A) qRT-PCR analysis of the expression levels of TCONS_00016233. (B and C) FCM analysis of HK-2 cell apoptosis. (D) Immunoblot analysis of cleaved caspase-3 and caspase-3. (E) Densitometric analysis of immunoblot bands. Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble with LPS or TCONS_00016233 siRNA group versus scramble with saline group; *p < 0.05, TCONS_00016233 siRNA with LPS group versus scramble with LPS group.