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. 2020 Feb 12;10:2405. doi: 10.1038/s41598-020-58369-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrographs of liver histology (A–C: Elastica-Masson staining, ×100; D–F: Binarized image, ×100; G: Quantification of liver fibrosis). The extent of fibrosis was quantified by binarizing to fibrous or not fibrous area using Win ROOF software (D: Untreated group; E: Shock wave group; F: Control group) in 10 microscopic fields (×100) per specimen in all rats. The quantified fibrous area was averaged for each group (G).