Table 1.

Specific inhibition of ALDH1A1 and SNAI2 (Slug) gene expressions in H1299-sdCSCs after treatment with EGCG and siAXL.

	Relative expression (fold of the control)†
	EGCG (10 μM)	EGCG (50 μM)	siControl	siAXL-1	siAXL-2
Stemness genes
CD133	1.07 ± 0.33	1.44 ± 0.01	1.64 ± 0.69	5.64 ± 0.83	1.70 ± 0.46
Nanog	1.05 ± 0.47	1.11 ± 0.47	1.14 ± 0.65	2.30 ± 1.24	1.35 ± 0.60
Sox2	0.70 ± 0.32	0.96 ± 0.08	1.32 ± 0.37	1.42 ± 0.65	2.08 ± 1.09
Oct4	1.01 ± 0.56	1.11 ± 0.39	1.12 ± 0.37	1.92 ± 1.16	1.25 ± 0.52
ALDH1A1	0.23 ± 0.12*	1.53 ± 1.32	1.23 ± 0.41	0.78 ± 0.87	0.31 ± 0.20*
EMT-marker genes
CDH2 (N-Cadherin)	1.26 ± 1.08	0.99 ± 0.46	2.23 ± 0.06	2.33 ± 0.89	2.03 ± 1.13
VIM (Vimentin)	1.00 ± 0.36	1.09 ± 0.27	1.07 ± 0.22	1.00 ± 0.04	1.03 ± 0.26
SNAI1(Snail)	1.14 ± 0.24	1.47 ± 0.29	1.05 ± 0.09	0.90 ± 0.41	0.87 ± 0.23
SNAI2 (Slug)	0.68 ± 0.18	0.20 ± 0.12*	1.42 ± 0.58	0.41 ± 0.24**	0.38 ± 0.19**
ZEB1	1.24 ± 0.25	1.09 ± 0.09	1.32 ± 0.21	1.25 ± 0.14	1.25 ± 0.30

^†Expression level of each gene normalized by GAPDH in control H1299-sdCSCs expressed as 1.0.

*P < 0.05, **P < 0.01.