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. 2020 Feb 12;10:2497. doi: 10.1038/s41598-020-59104-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Linear discriminant analysis (LDA) effect size (LEfSe; Kruskall-Wallis [P < 0.05]; Pairwise Wilcoxon [P < 0.05]; logarithmic LDA score > 2.0) of the fecal microbiota of (A) Angus steers before (Pre) placement or across a 26-day grazing trial after placement on either a non-toxic (Max-Q; n = 6) or toxic (E+; n = 6) endophyte-infected tall fescue; (B) steers grazing Max-Q tall fescue on Day 12 (- temperature humidity index [−THI]) versus Day 20 (+THI); (C) steers grazing E+ tall fescue on Day 12 (−THI) versus Day 20 (+THI). (A) Blue, green, and red shading indicates greater abundance in Pre, Max-Q, or E+ steers, respectively (C) Green and red indicates greater abundance in E+ steers on Day 12 (−THI) and 20 (+THI), respectively. Taxonomic rank labels are provided before bacterial names: “p_; c_; o_; f_; g_” indicate phylum, class, order, family, and genus, respectively. Letters and numbers within the cladogram refer to bacterial names located in the keys below (panel A) and to the right (panel C) of the cladogram.