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. 2020 Feb 12;10:2497. doi: 10.1038/s41598-020-59104-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Top 15 pathways from pathway analysis performed on the (A) plasma and (B) urine high-resolution metabolomics features using the mummichog python program within Day 12 (−THI) and 20 (+THI), with putative pathways that are significantly affected by toxic tall fescue (E+) grazing solely on Day 12 (−THI; orange), only on Day 20 (+THI; gray), or on both dates (plasma = red; urine = yellow). The Venn diagrams detail the number of putative metabolic pathways that were affected in the plasma and/or urine (top) or in the plasma on Day 12 and/or Day 20 and (bottom left) or in the urine on Day 12 and/or Day 20 (bottom right).