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. 2020 Feb 12;10:2497. doi: 10.1038/s41598-020-59104-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Coinertia analysis performed on OTUs for steers grazing either a (A; Max-Q; n = 6) novel, non-toxic or (B; E+; n = 6) toxic endophyte-infected tall fescue over the course of a 26-day grazing trial; Max-Q steers on Day 12 (C; lowest −THI) and Day 20 (E; highest +THI) and E+ steers on Day 12 (D; −THI) and Day 20 (F; +THI). Each point is plotted based on the OTUs predicted covariance with the plasma and urine metabolomes (i.e., greater distance from the point of origin indicates a greater covariance) of the respective group of steers. Red = Actinobacteria, brown = Bacteroidetes, green = Firmicutes, teal = other (unclassified, Planctomycetes, Chloroflexi, and other low abundance phyla), blue = Proteobacteria, purple = Tenericutes, and pink = Verrucomicrobia.