Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 22;30:101438. doi: 10.1016/j.redox.2020.101438

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 2 — Thioredoxin function supports Ahp1 urmylation. (A) Ahp1 oxidation by ROS (t-BOOH) and reduction by the thioredoxin system, i.e. NADPH-dependent thioredoxin reductase (Trr1) and thioredoxins (Trx1; Trx2). (B) EMSAs under reducing (left panels) and non-reducing (right panels) conditions with protein extracts of indicated strains expressing HA-URM1 (+) or not (−). Urmylation was studied by anti-HA (top panels) diagnostic for free HA-Urm1 and urmylated forms of Ahp1 (~36 kDa) or Ahp1 intersubunit disulfides (AID ~72 kDa). anti-Ahp1 blots (middle panels) detect unmodified Ahp1 (~19 kDa) and AIDs (~38 kDa). anti-Cdc19 (bottom panels) served as loading control. Asterisks denote faster migrating Ahp1 forms.