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. 2020 Jan 22;30:101438. doi: 10.1016/j.redox.2020.101438

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Suppression of Ahp1 urmylation and disulfide formation by t-BOOH in vivo. Shown are EMSAs under reducing (left panels) and non-reducing (right panels) conditions from strains treated with t-BOOH as indicated and expressing HA-URM1 (+) or not (−). NEM-stabilized HA-Urm1 conjugation was studied by anti-HA blots (top panels) diagnostic for free HA-Urm1 and urmylated forms of Ahp1 (~36 kDa) and Ahp1 intersubunit disulfides (AID ~72 kDa). anti-Ahp1 blots (middle panels) detect unmodified Ahp1 (~19 kDa) and AID (~38 kDa). anti-Cdc19 (bottom panels) served as internal standard.