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. 2019 Jul 23;10(2):358–373. doi: 10.1016/j.apsb.2019.07.004

Figure 4.

Figure 4

CRISPR-Cas9-mediated PD-L1 attenuation triggered T cell-mediated antitumor immune response in murine melanoma model. (A) Immunofluorescence images of tumor sections showed CD4+ T cells and CD8+ T cells infiltration. The scale bar is 100 μm. (B) Tumor weight of mice treated with PBS, naked pDNA, aPBAE/Cas9-null or aPBAE/Cas9-Cdk5 (n = 4). (C) Number of CD3+ cells per gram tumor after different treatments (n = 4). (D) Representative scatter plots of CD4+ T cells and CD8+ T cells in tumors (n = 4). (E) Ratios of CD8+ T cells and CD4+ T cells in tumors (n = 4). (F) Percentage of CD4+Foxp3+ cells in tumors (n = 4). PBS (G1), naked pDNA (G2), aPBAE/Cas9-null (G3) and aPBAE/Cas9-Cdk5 (G4). Data were expressed as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.005.