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. 2019 Jul 23;10(2):358–373. doi: 10.1016/j.apsb.2019.07.004

Figure 6.

Figure 6

Regulation of intrinsic mechanisms in 4T1 tumor after aPBAE/Cas9-Cdk5 treatment. (A) Light intensities analysis and (B) representative protein expression of Western blot for the breast carcinoma tissues dissected from mice treated with PBS, naked pDNA, aPBAE/Cas9-Cdk5 or anti-PD-L1 antibody (n = 3). (C) Heat map of the corresponding mRNA levels compared to GAPDH analyzed by qRT-PCR. (D) mRNA fold change of Cdk5, (E) p35 and (F) PD-L1 for the tumor tissues dissected from mice treated with PBS, naked pDNA, aPBAE/Cas9-Cdk5 or anti-PD-L1 antibody (n = 3). PBS (G1), naked pDNA (G2), aPBAE/Cas9-Cdk5 (G3) and anti-PD-L1 antibody (G4). (G) Immunofluorescence images of tumor sections showed CD8+ T cells and CD4+ T cells infiltration. The scale bar is 200 μm. (H) Representative IHC analysis of the expression of Cdk5 and PD-L1 after treatment. The scale bar is 50 μm. (I) TUNEL analysis in tumor sections after different treatments. The scale bar is 100 μm. Data were expressed as mean ± SD. ∗P < 0.05.